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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MKWVTFISLL FLFSSAYSRG VFRRDTYKSE IAHRFKDLGE QYFKGLVLIA FSQHLQQCPY

70 80 90 100 110 120
EEHVKLVREV TEFAKTCVAD ESAENCDKSI HTLFGDKLCA IPSLREHYGD LADCCEKEEP

130 140 150 160 170 180
ERNECFLQHK NDNPDIPKLK PDPVALCADF QEDEQKFWGK YLYEIARRHP YFYAPELLYY

190 200 210 220 230 240
AIIYKDVFSE CCQAADKAAC LLPKIEHLRE KVLTSAAKQR LKCASIQKFG ERAFKAWSLA

250 260 270 280 290 300
RLSQRFPKAD FTEISKIVTD LAKVHKECCH GDLLECADDR ADLAKYICEN QDTISTKLKE

310 320 330 340 350 360
CCDKPLLEKS HCIAEAKRDE LPADLNPLEH DFVEDKEVCK NYKEAKHVFL GTFLYEYSRR

370 380 390 400 410 420
HPDYSVSLLL RIAKIYEATL EDCCAKEDPP ACYATVFDKF QPLVDEPKNL IKQNCELFEK

430 440 450 460 470 480
LGEYGFQNAL IVRYTKKVPQ VSTPTLVEVA RKLGLVGSRC CKRPEEERLS CAEDYLSLVL

490 500 510 520 530 540
NRLCVLHEKT PVSEKVTKCC TESLVNRRPC FSALTPDETY KPKEFVEGTF TFHADLCTLP

550 560 570 580 590 600
EDEKQIKKQT ALVELLKHKP HATEEQLRTV LGNFAAFVQK CCAAPDHEAC FAVEGPKFVI


EIRGILA

References and documentation are available.

Please note the modified algorithm for extinction coefficient.


Number of amino acids: 607 Molecular weight: 69692.1 Theoretical pI: 6.08
Amino acid composition: 
Ala (A) 51 8.4% Arg (R) 29 4.8% Asn (N) 13 2.1% Asp (D) 36 5.9% Cys (C) 35 5.8% Gln (Q) 20 3.3% Glu (E) 61 10.0% Gly (G) 17 2.8% His (H) 19 3.1% Ile (I) 24 4.0% Leu (L) 65 10.7% Lys (K) 58 9.6% Met (M) 1 0.2% Phe (F) 33 5.4% Pro (P) 30 4.9% Ser (S) 27 4.4% Thr (T) 27 4.4% Trp (W) 3 0.5% Tyr (Y) 23 3.8% Val (V) 35 5.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 97 Total number of positively charged residues (Arg + Lys): 87 Atomic composition: Carbon C 3119 Hydrogen H 4876 Nitrogen N 826 Oxygen O 912 Sulfur S 36 Formula: C3119H4876N826O912S36 Total number of atoms: 9769 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 52895 Abs 0.1% (=1 g/l) 0.759, assuming ALL Cys residues appear as half cystines Ext. coefficient 50770 Abs 0.1% (=1 g/l) 0.728, assuming NO Cys residues appear as half cystines Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 40.99 This classifies the protein as unstable. Aliphatic index: 82.31 Grand average of hydropathicity (GRAVY): -0.370

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